Mouse Monoclonal IgG1 Anti-Collagen Type VII, human [LH7.2]

• Catalog number
  COL007
• Price
  Please ask
• Size
  1 ml

• Availability
  Please contact us to check the availability
• Immunogen
  Extract of human neonatal foreskin
• Specificity
  Collagen type VII, carboxyterminal
• Species reactivity
  Human, Marmoset, Monkey, Cow, Sheep, Goat, and Guinea pig
• Product presentation
  Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
• Product category
  Primary Antibody
• Antibody s raised in
  Mouse
• Antibody s isotype
  IgG1
• Antibody s clone
  LH7.2
• Product is supplied as
  liquid concentrate
• Recommended concentration for use
  1:10 - 1:20 IHC(C)
• Protein concentration
  50 µg/ml
• Positive control
  Normal skin
• Pretreatment
  Fixation with ice cold Acetone only (Methanol is not suitable!) No method known for paraffin sections!
• Tested applications
  IHC(C), IFA
• Recommended secondary reagent
  Anti-mouse IgG-conjugates, e.g. Anti-mouse IgG:FITC (Art. No. FI-2000) or anti-mouse IgG:DyLight488 (Art. No. DI-2488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7402).
• Storage method
  Store at 2-8°C
• UniProt number
  QGo to UniProt/SwissProt2388 (CO7A1_HUMAN)
• Litterature
  1. Hegerty A.M.H., et al. (1986) Identification of an epidermal basement membrane defect in recessive forms of dystrophic epidermolysis bullosa by LH7.2 monoclonal antibody: use in diagnosis. Br. J. Dermatol. 115; 125-131. 2. Leigh IM, Eady RA, Heagerty AH, Purkis PE, Whitehead PA, Burgeson RE (1988) Type VII collagen is a normal component of epidermal basement membrane, which shows altered expression in recessive dystrophic epidermolysis bullosa. J Invest Dermatol. 90(5): 3. Kirkham N., et al. (1989) Type VII collagen antibody, LH7.2 identifies basement membrane charakteristics of thin malignanat melanomas. J. Pathol. 157; 243-247.
• Tips
  *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
• Other products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Gene
  Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Monoclonal   IgG1   Collagen   VII   [LH7  
• Gene symbol
  MARCHF7, IGLVVII-41-1, SYT7
• Short name
  ECM
• Technique
  Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Isotype
  IgG1
• Species
  Human, Humans, Mouses
• Alternative name
  Collagen Type VII, human
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:17393
• Gene
  MARCHF7
• Long gene name
  membrane associated ring-CH-type finger 7
• Synonyms gene
  • AXOT
  • MARCH7
• Synonyms gene name
  • axotrophin
  • membrane-associated ring finger (C3HC4) 7
  • membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase
• Synonyms
  • MARCH-VII
  • RNF177
• GenBank acession
  • AK022973
• Locus
  2q24.2
• Discovery year
  2001-12-17
• Entrez gene record
  64844
• Pubmed identfication
  • 14722266
• RefSeq identity
  • NM_022826
• Classification
  • Ring finger proteins
  • Membrane associated ring-CH-type fingers
• VEGA ID
  OTTHUMG00000132029

Gene info

• Identity
  HGNC:15691
• Gene
  IGLVVII-41-1
• Long gene name
  immunoglobulin lambda variable (VII)-41-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable (VII)-41-1
  • immunoglobulin lambda variable (VII)-41-1 pseudogene
• Synonyms
  • IGLV(VII)-41-1
• GenBank acession
  • X99568
• Locus
  22q11.22
• Discovery year
  2001-05-15
• Entrez gene record
  84089
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151296

Gene info

• Identity
  HGNC:11514
• Gene
  SYT7
• Long gene name
  synaptotagmin 7
• Synonyms gene
  • PCANAP7
• Synonyms gene name
  • prostate cancer associated protein 7
  • synaptotagmin VII
• Synonyms
  • IPCA-7
  • SYT-VII
  • MGC150517
• GenBank acession
  • AF038535
• Locus
  11q12.2
• Discovery year
  1999-07-14
• Entrez gene record
  9066
• Pubmed identfication
  • 9615227
• RefSeq identity
  • NM_004200
• Classification
  • Synaptotagmins
• VEGA ID
  OTTHUMG00000168199

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products