Purified human Collagen IV
Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate
Human and other primates (no reactivity in chicken, cat, rabbit, rat, sheep and goat)
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
Product is supplied as
Recommended concentration for use
1:10 - 1:50
app. 100 µg/ml
Human skin, placenta or lens capsule
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Store at 2-8°C
PGo to UniProt/SwissProt2462 (CO4A1_HUMAN) ; PGo to UniProt/SwissProt8572 (CO4A2_HUMAN); P294Go to UniProt/SwissProtGo to UniProt/SwissProt (CO4A5_HUMAN);
1. Odermatt B.F., Lang A.B., Rüttner J.R., Winterhalter K.H., and Trüeb B. (1984) Monoclonal antibodies to human type IV collagen: useful reagents to demonstate the heterotrimeric nature of the molecule. Proc. Natl. Acad. Sci. 81; 7343-7347.
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!