Mouse anti Cytokeratin 13 / Keratin K13

• Catalog number
  MUB0322P
• Price
  Please ask
• Size
  0,1 mg

• Category
  Primary Antibodies
• Long description
  Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
• Antibody come from
  1C7 is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a Cytokeratin preparation extracted from Human esophagus.
• Other description
  Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Clone
  1C7
• Antigen antibody binding interaction
  Mouse anti Cytokeratin 13 / Keratin K13 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human,Zebrafish
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  1C7 reacts exclusively with Cytokeratin 13 which is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stRatified epithelia.
• Research interest
  Cytoskeleton
• Application
  Immunohistochemistry (frozen),Western blotting
• Antibody s suited for
  1C7 is suitable for immunoblotting and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  van Muijen, G. N., Ruiter, D. J., Franke, W. W., Achtstatter, T., Haasnoot, W. H., Ponec, M., and Warnaar, S. O. (1986). Cell type heterogeneity of Cytokeratin expression in complex epithelia and carcinomas as demonstRated by monoclonal antibodies specific for Cytokeratins nos. 4 and 13, Exp Cell Res 162, 97-113. Weikel, W., Wagner, R., and Moll, R. (1987). Characterization of subcolumnar reserve cells and other epithelia of Human uterine cervix. DemonstRation of diverse Cytokeratin polypeptides in reserve cells, Virchows Arch B Cell Pathol Incl Mol Pathol 54, 98-110.Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of Keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68.van Niekerk, C. C., Boerman, O. C., Ramaekers, F. C., and Poels, L. G. (1991). Marker profile of different phases in the transition of normal Human ovarian epithelium to ovarian carcinomas, Am J Pathol 138, 455-63.Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Link, M., Lane, B., Leigh, I., Schijf, C., and Vooijs, P. (1992). Keratin expression in cervical cancer, Am J Pathol 141, 497-511.Bauwens, L. J., De Groot, J. C., Ramaekers, F. C., Veldman, J. E., and Huizing, E. H. (1992). Expression of intermediate filament proteins in the adult Human vestibular labyrinth, Ann Otol Rhinol Laryngol 101, 479-86.Van Niekerk, C. C., Ramaekers, F. C., Hanselaar, A. G., Aldeweireldt, J., and Poels, L. G. (1993). Changes in expression of differentiation markers between normal ovarian cells and derived tumors, Am J Pathol 142, 157-77.van Dorst, E. B., van Muijen, G. N., Litvinov, S. V., and Fleuren, G. J. (1998). The limited difference between Keratin patterns of squamous cell carcinomas and adenocarcinomas is explicable by both cell lineage and state of differentiation of tumour cells, J Clin Pathol 51, 679-84.
• Protein number
  P13646
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Cytokeratin   Keratin   K13  
• Gene symbol
  KRT13, KCNG1
• Short name
  Mouse anti Cytokeratin 13 / Keratin K13
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG2a
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Cytokeratin 13 / Keratin K13
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:6415
• Gene
  KRT13
• Long gene name
  keratin 13
• Synonyms gene name
  • keratin 13, type I
• Synonyms
  • K13
  • CK13
  • MGC3781
  • MGC161462
• Synonyms name
  • keratin, type I cytoskeletal 13
  • cytokeratin 13
• Locus
  17q21.2
• Discovery year
  1990-11-05
• Entrez gene record
  3860
• Pubmed identfication
  • 16831889
• RefSeq identity
  • NM_153490
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133434

Gene info

• Identity
  HGNC:6248
• Gene
  KCNG1
• Long gene name
  potassium voltage-gated channel modifier subfamily G member 1
• Synonyms gene
  • KCNG
• Synonyms gene name
  • potassium voltage-gated channel, subfamily G, member 1
• Synonyms
  • Kv6.1
  • kH2
  • K13
• GenBank acession
  • AF033383
• Locus
  20q13.13
• Discovery year
  1993-03-22
• Entrez gene record
  3755
• Pubmed identfication
  • 9434767
  • 16382104
• RefSeq identity
  • NM_002237
• Classification
  • Potassium voltage-gated channels
• VEGA ID
  OTTHUMG00000032745

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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