CD80 monoclonal antibody
-
Catalog number11-627-C100
-
PricePlease ask
-
Size100ug
-
-
Long nameHamster Monoclonal Antibody against Mouse CD80, Clone: 16-10A1
-
AntigenCD80
-
Species reactivityMouse (Mus musculus), Canine (Dog)
-
Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
-
ClonalityMonoclonal Antibody (Mab)
-
Clone16-10A1
-
ApplicationsFC, IP, IHC(F), FUNC
-
ImmunogenCD80-transfected CHO cell line_x000D_
-
Format conjugationpurified; unconjugated
-
SpecificityThe Armenian hamster monoclonal antibody 16-10A1 reacts with CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes. _x000D_
-
Research areaCD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent)
-
Concentration1 mg/ml
-
Storage bufferPhosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
-
Shipping and storageHamster Monoclonal Antibody against Mouse CD80, Clone: 16-10A1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
-
BackgroundCD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response. _x000D_
-
Purity> 95% (by SDS-PAGE)
-
Purification methodPurified by protein-A affinity chromatography
-
References_x000D_ , *Mukherjee S, Ahmed A, Malu S, Nandi D: Modulation of cell cycle progression by CTLA4-CD80/CD86 interactions on CD4+ T cells depends on strength of the CD3 signal: critical role for IL-2. J Leukoc Biol. 2006 Jul;80(1):66-74._x000D_ , *Quah B, Ni K, O'Neill HC: In vitro hematopoiesis produces a distinct class of immature dendritic cells from spleen progenitors with limited T cell stimulation capacity. Int Immunol. 2004 Apr;16(4):567-77._x000D_ , *Paust S, Lu L, McCarty N, Cantor H: Engagement of B7 on effector T cells by regulatory T cells prevents autoimmune disease. Proc Natl Acad Sci U S A. 2004 Jul 13;101(28):10398-403._x000D_ , *Reiser J, von Gersdorff G, Loos M, Oh J, Asanuma K, Giardino L, Rastaldi MP, Calvaresi N, Watanabe H, Schwarz K, Faul C, Kretzler M, Davidson A, Sugimoto H, Kalluri R, Sharpe AH, Kreidberg JA, Mundel P: Induction of B7-1 in podocytes is associated with nephrotic syndrome. J Clin Invest. 2004 May;113(10):1390-7._x000D_ , *Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, Holdsworth SR: Glomerular expression of CD80 and CD86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis. J Am Soc Nephrol. 2005 Jul;16(7):2012-22._x000D_ , *Mencacci A, Montagnoli C, Bacci A, Cenci E, Pitzurra L, Spreca A, Kopf M, Sharpe AH, Romani L: CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis. J Immunol. 2002 Sep 15;169(6):3180-90._x000D_ , *Horspool JH, Perrin PJ, Woodcock JB, Cox JH, King CL, June CH, Harlan DM, St Louis DC, Lee KP: Nucleic acid vaccine-induced immune responses require CD28 costimulation and are regulated by CTLA4. J Immunol. 1998 Mar 15;160(6):2706-14._x000D_ , *Gueirard P, Ave P, Balazuc AM, Thiberge S, Huerre M, Milon G, Guiso N: Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract. Infect Immun. 2003 Jul;71(7):4137-43._x000D_
-
PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
French translationanticorps
-
Gene target
-
Gene symbolCD80
-
Short nameAnti-CD80 Mab
-
TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
-
IsotypeIgG2
-
Alternative nameHamster Monoclonal to CD80
-
Alternative techniqueantibodies
-
Alternative to gene targetCD80 molecule, B7 and B7-1 and B7.1 and BB1 and CD28LG and CD28LG1 and LAB7, CD80 and IDBG-51287 and ENSG00000121594 and 941, coreceptor activity, Cell surfaces, Cd80 and IDBG-160288 and ENSMUSG00000075122 and 12519, BT.27986 and IDBG-632750 and ENSBTAG00000018059 and 407131
-
Gene info
-
Identity
-
Gene
-
Long gene nameCD80 molecule
-
Synonyms gene
-
Synonyms gene name
- CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)
- CD80 molecule
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1993-12-14
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- B7 family
- CD molecules
- C2-set domain containing
- V-set domain containing
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data