RANKL Antibody

• Catalog number
  R32452
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  RANKL
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, ELISA (protein)
• Recommended dilutions
  Western blot: 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml (mouse protein tested); request BSA-free format for coating
• Added buffer
  Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
• Intented use
  This RANKL antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9ESE2
• Purity
  Antigen affinity
• Description
  Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. It is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
• Immunogen
  Amino acids F141-D318 from the rat protein were used as the immunogen for the RANKL antibody.
• Storage
  Prior to reconstitution, store at 4oC. After reconstitution, the RANKL antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  RANKL  
• Gene symbol
  TNFSF11
• Short name
  Anti- RANKL
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to RANKL
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:11926
• Gene
  TNFSF11
• Long gene name
  TNF superfamily member 11
• Synonyms gene name
  • tumor necrosis factor (ligand) superfamily, member 11
• Synonyms
  • TRANCE
  • RANKL
  • OPGL
  • ODF
  • CD254
• GenBank acession
  • AF013171
• Locus
  13q14
• Discovery year
  1998-12-04
• Pubmed identfication
  • 9312132
  • 9367155
• Classification
  • Tumor necrosis factor superfamily
  • CD molecules
• VEGA ID
  OTTHUMG00000016807

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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