GDF-15 (Human) ELISA Kit
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Catalog numberE4887-100
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PricePlease ask
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Size96 assays
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DescriptionA Sandwich ELISA kit to quantitatively measure GDF-15 in human serum, plasma, tissue lysates, and other biological fluids
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Detection MethodAbsorbance (450 nm)
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Species ReactivityHuman
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ApplicationsThis Sandwich ELISA kit is designed to quantitatively measure amount of GDF-15 in human serum, plasma, tissue lysates, and other biological fluids
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Sample TypePlasma,Serum,Tissue lysate,Other biological fluids
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Features BenefitsSensitivity: 14.063 pg/ml,Recovery range: 85 - 105% for normal human serum and plasma samples,This Sandwich ELISA is highly sensitive and highly specific for the detection of GDF-15 in human samples. There is no significant cross-reactivity or interference of GDF-15 with analogues,Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%,Detection range: 23.438 - 1500 pg/ml
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Storage Conditions4ºC
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Shipping ConditionsGel Pack
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Shelf life6 months
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BackgroundGrowth Differentiation Factor 15 (GDF-15), also known as Macrophage Inhibitory Cytokine-1 (MIC-1), belongs to the superfamily of transforming growth factor-beta (TGF-β). During normal conditions, GDF-15 is expressed at low levels in organs such as kidney, stomach, bladder, and placenta. Under stress conditions, the expression is upregulated by various stress-related proteins such as IL-1β, TNF-α, IL-2 in a tissue-specific manner. Elevated expressions are observed in diseased states such as cancer, inflammation, cardiovascular disease, and kidney disease. Hence, GDF-15 serves as an efficient biomarker for cancer and cardiovascular disease. BioVision’s GDF-15 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of GDF-15 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added, and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of GDF-15 in the sample or standard.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbolPIRC66, PIRC71, PIRC75, PIRC67, PIRC72, PIRC68, PIRC62, PIRC70, PIRC76, PIRC77
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Short nameGDF-15 ( ) ELISA Kit
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TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Hostantigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesHuman, Humans
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Alternative nameGDF-15 (H. sapiens) Enzyme-linked immunosorbent assay reagent
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Alternative techniqueelisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 66
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 71
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 75
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 67
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 72
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 68
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 62
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 70
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 76
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Locus
-
Discovery year2009-11-05
-
Entrez gene record
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Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
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Long gene namepiwi-interacting RNA cluster 77
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Locus
-
Discovery year2009-11-05
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Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data