Aldolase A, human recombinant

• Catalog number
  7359-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Fructose bisphosphate Aldolase A, ALDOA, ALDA, GSD12
• Alternative_names
  Fructose bisphosphate Aldolase A, ALDOA, ALDA, GSD12
• Description
  Catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Molecular Weight
  41.5 kDa (384 aa, 1-364 aa + His Tag), confirmed by MALDI-TOF.
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl and 10% glycerol.
• Background Information
  Fructose bisphosphate aldolase A, also known as Aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. It is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Deficiency has been associated with myopathy and hemolytic anemia. Recombinant human Aldolase A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MPYQYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE NTEENRRFYR QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS KGGVVGIKVD KGVVPLAGTN GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKIGEHTPS ALAIMENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HIYLEGTLLK PNMVTPGHAC TQKFSHEEIA MATVTALRRT VPPAVTGITF LSGGQSEEEA SINLNAINKC PLLKPWALTF SYGRALQASA LKAWGGKKEN LKAAQEEYVK RALANSLACQ GKYTPSGQAG AAASESLFVS NHAY
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Aldolase  
• Gene symbol
  ALDOA, ALDOAP1, ALDOAP2
• Short name
  Aldolase A, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Aldolase A, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:414
• Gene
  ALDOA
• Long gene name
  aldolase, fructose-bisphosphate A
• Synonyms gene name
  • aldolase A, fructose-bisphosphate
• GenBank acession
  • X05236
• Locus
  16p11.2
• Discovery year
  1986-01-01
• Entrez gene record
  226
• Pubmed identfication
  • 3570299
• RefSeq identity
  • NM_000034
• Classification
  • Aldolases
• VEGA ID
  OTTHUMG00000132107
• Locus Specific Databases
  • LRG_1180

Gene info

• Identity
  HGNC:415
• Gene
  ALDOAP1
• Long gene name
  ALDOA pseudogene 1
• Synonyms gene name
  • aldolase A, fructose-bisphosphate pseudogene 1
  • aldolase, fructose-bisphosphate A pseudogene 1
• Locus
  3p21.2
• Discovery year
  1986-01-01
• Entrez gene record
  391538
• VEGA ID
  OTTHUMG00000157814

Gene info

• Identity
  HGNC:416
• Gene
  ALDOAP2
• Long gene name
  ALDOA pseudogene 2
• Synonyms gene name
  • aldolase A, fructose-bisphosphate pseudogene 2
  • aldolase, fructose-bisphosphate A pseudogene 2
• GenBank acession
  • M21191
• Locus
  10q26.13
• Discovery year
  1986-01-01
• Entrez gene record
  228
• Pubmed identfication
  • 3674018
• VEGA ID
  OTTHUMG00000019228

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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