CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)

• Catalog number
  K163-100
• Price
  Please ask
• Size
  100 assays

• Description
  Specifically detects Caspase-3
• Summary
  • Detection method- Fluorescence (Ex/Em 370-425/ 490-525 nm) • Species reactivity- Mammalian • Kit size- 100 assays • Applications- Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates.
• Sample Type
  Cell and tissue lysates
• Features Benefits
  • Simple one-step procedure; takes only ~3 hours • Fast and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  6-12 months
• Background
  Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.
• Data Sheet Link
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

• Gene target
  CaspSELECT™   Caspase-3   Immunoassay   Kit   Fluorometric  
• Short name
  CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)
• Technique
  immunoassay
• Alternative name
  CaspSELECT™ caspase-3 Immunoassay reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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