Anti-TFIID/TATA binding protein TBP, ALEXA Fluor 594

• Catalog number
  GENTObs-11766R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  TFIID/TATA binding protein TBP
• Specificity
  This antibody reacts specifically with TFIID/TATA binding protein TBP
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human TATA binding protein TBP
• Gene ID
  N/A
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Binding of TFIID to the TATA element initiates assembly of the other factors into a pre-initiation complex. The TATA-binding subunit of TFIID (designated TFIIDt or TBP) from higher eukaryotes contains a highly conserved 180 amino acid C-terminal domain.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  GTF2D; GTF2D1; MGC117320; MGC126054; MGC126055; SCA 17; SCA17; TATA binding factor; TATA box binding protein; TATA box factor; TATA sequence binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; TATA-box-binding protein; TBP; TBP_HUMAN; TF2D; TFIID; Transcription initiation factor TFIID TBP subunit.
• Also known as
  TFIID/TATA binding protein TBP Antibody
• Other name
  Anti-TFIID/TATA binding protein TBP
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.

• Gene target
  TFIID/TATA   binding   protein   TBP  
• Gene symbol
  TBP, PSMC3, TAF9B, PSMC4
• Short name
  Anti-TFIID/TATA binding protein TBP
• Technique
  anti-, anti, antibody to, antibodies
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  TFIID/TATA binding protein TBP Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:11588
• Gene
  TBP
• Long gene name
  TATA-box binding protein
• Synonyms gene
  • GTF2D1
  • SCA17
• Synonyms
  • TFIID
• GenBank acession
  • M55654
• Locus
  6q27
• Discovery year
  1993-05-26
• Entrez gene record
  6908
• Pubmed identfication
  • 2194289
  • 11448935
• RefSeq identity
  • NM_003194
• Classification
  • General transcription factor IID complex subunits
  • General transcription factor IIIB complex subunits
  • General transcription factors
• VEGA ID
  OTTHUMG00000016084

Gene info

• Identity
  HGNC:9549
• Gene
  PSMC3
• Long gene name
  proteasome 26S subunit, ATPase 3
• Synonyms gene name
  • proteasome (prosome, macropain) 26S subunit, ATPase, 3
• Synonyms
  • TBP1
  • TBP-1
  • RPT5
• GenBank acession
  • M34079
• Locus
  11p11.2
• Discovery year
  1995-12-06
• Entrez gene record
  5702
• Pubmed identfication
  • 9048938
  • 9473509
• RefSeq identity
  • NM_002804
• Classification
  • Proteasome
  • AAA ATPases
• VEGA ID
  OTTHUMG00000167692

Gene info

• Identity
  HGNC:17306
• Gene
  TAF9B
• Long gene name
  TATA-box binding protein associated factor 9b
• Synonyms gene
  • TAF9L
• Synonyms gene name
  • TAF9-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
  • TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
• Synonyms
  • TAFII31L
  • DN-7
  • DN7
  • TFIID-31
• GenBank acession
  • AF220509
• Locus
  Xq21.1
• Discovery year
  2001-12-03
• Entrez gene record
  51616
• Pubmed identfication
  • 15899866
• RefSeq identity
  • NM_015975
• VEGA ID
  OTTHUMG00000021887
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:9551
• Gene
  PSMC4
• Long gene name
  proteasome 26S subunit, ATPase 4
• Synonyms gene
  • MIP224
• Synonyms gene name
  • proteasome (prosome, macropain) 26S subunit, ATPase, 4
• Synonyms
  • TBP7
  • S6
  • MGC8570
  • MGC13687
  • MGC23214
  • TBP-7
  • RPT3
• Synonyms name
  • protease 26S subunit 6
  • Tat-binding protein 7
  • MB67 interacting protein
• GenBank acession
  • U27515
• Locus
  19q13.11-q13.13
• Discovery year
  1995-12-06
• Entrez gene record
  5704
• Pubmed identfication
  • 9473509
  • 8603043
• RefSeq identity
  • NM_006503
• Classification
  • AAA ATPases
  • Proteasome
• VEGA ID
  OTTHUMG00000182577

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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