ATG16 Antibody (Center)
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Catalog number5070-100
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PricePlease ask
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Size100 µg
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Antibody TargetATG16
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Target Alternative NameAutophagy protein 16, Autophagy related protein 16, ATG16L, APG16
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceATG16 antibody was raised against a 18 amino acid peptide from near the center of human ATG16.
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AccessionQ676U5
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Gene Id55054
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AppearanceColorless liquid
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FormLiquid
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Concentration1 mg/ml
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Formulation100 µg (1 mg/ml) in 1X PBS containing 0.02% sodium azide.
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PurificationAffinity chromatography purified via peptide column.
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ApplicationWestern blot, Immunocytochemistry, ELISA
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Positive ControlWestern Blot: HeLa cell lysate Immunocytochemistry: HeLa cells
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Application And UsagesWestern Blot: 0.25 – 0.5 μg/ml, Immunocytochemistry: 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.
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DescriptionAutophagy, the process of bulk degradation of cellular proteins throμgh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) throμgh phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjμgate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been sμggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedVerified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short nameATG16 Antibody (Center)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG1
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Alternative nameATG16 (antibody to-) (central)
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data