Human Recombinant AKR7A2

• Catalog number
  6335-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Human Recombinant AKR7A2
• Alternative_names
  Aldo-keto reductase family 7, member A 2, Aflatoxin B1 aldehyde reductase member 2, AFAR, AFAR1, AFB1-AR1, AKR7.
• Description
  Involved in in the detoxification of aldehydes and ketones
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is approximately 0.25 – 0.3 units/mg.
• Unit Definition
  Specific activity is approximately 0.25 – 0.3 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Specific activity was expressed as units/mg protein.
• Molecular Weight
  44 kDa
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 20% glycerol.
• Background Information
  Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones. This protein can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the nonbinding AFB1 dialcohol. It may be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Also, it has been proposed previously to catalyze the NADPH-dependent reduction of succinic semialdehyde (SSA) to Gamma-Hydroxybutyrate in human brain.
• Amino acid sequence
  MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELE LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  AKR7A2  
• Gene symbol
  AKR7A2
• Short name
  Recombinant AKR7A2
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. AKR7A2
• Alternative technique
  rec

Gene info

• Identity
  HGNC:389
• Gene
  AKR7A2
• Long gene name
  aldo-keto reductase family 7 member A2
• Synonyms
  • AFAR
• Synonyms name
  • aflatoxin aldehyde reductase
• GenBank acession
  • AF026947
• Locus
  1p36.13
• Discovery year
  1998-11-17
• Entrez gene record
  8574
• Pubmed identfication
  • 9576847
• RefSeq identity
  • NM_003689
• Classification
  • Aldo-keto reductases
• VEGA ID
  OTTHUMG00000002522

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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