This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
200µg (1 mg/ml) in 1X PBS, pH 7.4 with 50% Glycerol
Alternative names of antibody target:
GFP antibody, Green Fluorescent Protein antibody, Enhanced green Fluorescent antibody
Western blotting (0.5-10 µg/ml).
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Clonality of antibody:
Protein G purified
Recombinant EGFP expressed in E. coli (Cat. # 4999-100)
How to handle this antibody:
The antibody solution should be gently mixed before use.
EGFP is an improved variant of GFP (green fluorescent protein) with intense green fluorescence. It is an ideal tool for monitoring the dynamics of tagged proteins for protein-protein integration, cell-sorting, organelle labeling, protein localization, gene expression and more. The anti-eGFP antibody recognizes over expressed recombinant proteins containing the eGFP tag fused to either the amino- or carboxy termini of targeted proteins in transfected mammalian cells or other expression systems.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
anti-GFP mAntibody (Clone 1)
antibody to-Green Fluorescent mAntibody (clonality 1)