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Nanog TAT human recombinant

Nanog TAT human recombinant is available 2 times from Biovision labs

7177-50 | Nanog-TAT, human recombinant size: 50 ug | 877.73 USD

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7177-10 | Nanog-TAT, human recombinant size: 10 ug | 250.24 USD

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Synonyms Homeobox transcription factor Nanog
Alternative_names Homeobox transcription factor Nanog
Description A regulatory protein associated with undifferentiated pluripotent cells
Recombinant Yes
Source E. Coli
Purity by SDS-PAGE ≥98%
Assay SDS-PAGE
Endotoxin Level< 0.1 ng/ug of protein (
Molecular Weight 36.2 kDa
Storage Temp. -20°C
Shipping Gel Pack
Shelf Life 12 months
Appearance Lyophilized powder
Physical form description Sterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Sodium Citrate pH 3.0 and 100 mM NaCl.
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Background Information Nanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of Nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of Nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, which helps maintain pluripotency and self-renewal via a STAT3 independent pathway. The introduction of Nanog, along with Sox2, Oct4, Lin28, into primary human fibroblasts was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology and gene expression. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human Nanog-TAT is a 36.2 kDa protein, which is synthesized as a 304 amino acid polypeptide plus a 13- residue C-terminal TAT peptide.
Amino acid sequence MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA ENSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDVGGYGR KKRRQRRR
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetNanog-TAT
Short name Nanog-TAT, recombinant
Technique Recombinant
Species Human
Alternative name Nanog-TAT, H. sapiens recombinant
Alternative technique rec
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