Mouse anti Interleukin-1 alpha (IL-1 a) antibody conjugated to FITC
-
Catalog numberL121M
-
PricePlease ask
-
Size100 Tests
-
-
CategoryPrimary Antibodies
-
Long descriptionRecognizes human IL-1 alpha. (Does not recognize mouse IL-1 alpha (human IL-1 beta or a panel of other human cytokines.
-
Antibody come fromHuman recom- binant interleukin 1 alpha (hIL-1 ).
-
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
-
CloneAS5
-
Antigen antibody binding interactionMouse anti Interleukin-1 alpha (IL-1 a) antibody conjugated to FITC Antibody
-
Antibody is raised inMouse
-
Antibody s reacts withHuman
-
Antibody s reacts with these speciesThis antibody doesn't cross react with other species
-
Antibody s specificityNo Data Available
-
ApplicationFlow Cytometry
-
Antibody s suited forPBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
-
Storage-20ºC
-
Relevant referencesno information yet
-
Protein numbersee ncbi
-
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
DescriptionThe Mouse anti Interleukin-1 alpha (IL-1 a) antibody conjugated FITC is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
PropertiesIf you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
-
ConjugationAnti-FITC Antibody
-
GeneThe Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
French translationanticorps
-
Gene target
-
Gene symbolIL1A, IL1B, IL1F10, IL36B, IL36A, IL19, IL17F, IL36G, IL25, IL22
-
Short nameMouse anti Interleukin-1 alpha (IL-1 a) antibody conjugated FITC
-
TechniqueAntibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
-
Hostmouse
-
IsotypeIgG1
-
LabelFITC
-
SpeciesMouse, Mouses
-
Alternative nameMouse antibody to Interleukin-1 a (Interleukin-1 a) (antibody to-) coupled to fluorecein
-
Alternative techniqueantibodies, murine, fluorescine
-
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 1 alpha
-
Synonyms gene
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 1 beta
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1989-03-31
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 1 family member 10
-
Synonyms gene name
- interleukin 1 family, member 10 (theta)
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2002-08-02
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 36 beta
-
Synonyms gene
-
Synonyms gene name
- interleukin 1 family, member 8 (eta)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2002-08-02
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 36 alpha
-
Synonyms gene
-
Synonyms gene name
- interleukin 1 family, member 6 (epsilon)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2002-08-02
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 19
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2000-03-29
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 17F
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2002-03-18
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 36 gamma
-
Synonyms gene
-
Synonyms gene name
- interleukin 1 family, member 9
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2002-08-02
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 25
-
Synonyms gene
-
Synonyms gene name
- interleukin 17E
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2001-01-26
-
Pubmed identfication
-
Classification
- Interleukins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 22
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2001-03-12
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data