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LIF human recombinant

LIF human recombinant is available 3 times from Biovision labs

4923-50 | LIF, human recombinant size: 50 ug | 725.80 USD

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4923-10 | LIF, human recombinant size: 10 ug | 337.80 USD

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4923-1000 | LIF, human recombinant size: 1 mg | 6,175.41 USD

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Synonyms Leukemia Inhibitory Factor
Alternative_names Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor, INN=Emfilermin
Description A cytokine that affects cell growth by inhibiting differentiation
Recombinant Yes
Source E. coli
Purity by SDS-PAGE ≥95%
Assay SDS-PAGE
Purity ≥95%
Biological activity The ED₅₀ determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of 100MIU/mg.
Molecular Weight 19.7 kDa
Storage Temp. -20°C
Shipping Gel pack
Shelf Life 12 months
Appearance Lyophilized protein
Physical form description Lyophilized from a concentrated (1 mg/ml) sterile solution containing 1x PBS.
Reconstitution Instructions Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Background Information Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant human LIF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.7 kDa.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetLIF
Short name LIF, recombinant
Technique Recombinant
Species Human
Alternative name leukemia inhibitory factor, H. sapiens recombinant
Alternative technique rec
Alternative to gene target leukemia inhibitory factor, CDF and DIA and HILDA and MLPLI, LIF and IDBG-3968 and ENSG00000128342 and 3976, growth factor activity, Extracellular, Lif and IDBG-142317 and ENSMUSG00000034394 and 16878, LIF and IDBG-636335 and ENSBTAG00000007424 and 280840
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