LIF, human recombinant

• Catalog number
  4923-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Leukemia Inhibitory Factor
• Alternative_names
  Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor, INN=Emfilermin
• Description
  A cytokine that affects cell growth by inhibiting differentiation
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Purity
  ≥95%
• Biological activity
  The ED₅₀ determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of 100MIU/mg.
• Molecular Weight
  19.7 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Lyophilized from a concentrated (1 mg/ml) sterile solution containing 1x PBS.
• Reconstitution Instructions
  Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
• Background Information
  Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant human LIF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.7 kDa.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  LIF  
• Gene symbol
  LIF-AS1, LIF-AS2, LIF
• Short name
  LIF, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  leukemia inhibitory factor, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  leukemia inhibitory factor, CDF and DIA and HILDA and MLPLI, LIF and IDBG-3968 and ENSG00000128342 and 3976, growth factor activity, Extracellular, Lif and IDBG-142317 and ENSMUSG00000034394 and 16878, LIF and IDBG-636335 and ENSBTAG00000007424 and 280840

Gene info

• Identity
  HGNC:53473
• Gene
  LIF-AS1
• Long gene name
  LIF antisense RNA 1
• Synonyms
  • Lnc-LIF-AS
• Locus
  22q12.2
• Discovery year
  2017-04-28
• Pubmed identfication
  • 28391028
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:28298
• Gene
  LIF-AS2
• Long gene name
  LIF antisense RNA 2
• Locus
  22q12.2
• Discovery year
  2020-10-07
• Entrez gene record
  91370
• RefSeq identity
  • NR_148946
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:6596
• Gene
  LIF
• Long gene name
  LIF interleukin 6 family cytokine
• Synonyms gene name
  • leukemia inhibitory factor
  • LIF, interleukin 6 family cytokine
• Synonyms
  • CDF
  • DIA
  • HILDA
• Synonyms name
  • differentiation inhibitory activity
  • differentiation-inducing factor
  • hepatocyte-stimulating factor III
  • cholinergic differentiation factor
  • human interleukin in DA cells
• Locus
  22q12.2
• Discovery year
  1989-06-11
• Entrez gene record
  3976
• Pubmed identfication
  • 1714745
  • 8058719
• RefSeq identity
  • NM_002309
• Classification
  • Receptor ligands
  • Interleukin 6 type cytokine family
• VEGA ID
  OTTHUMG00000150910
• Locus Specific Databases
  • ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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