Synonyms Insulin-like Growth Factor-Binding Protein 3, Growth-hormone-dependant binding protein
Alternates names Insulin-like Growth Factor-Binding Protein 3, Growth-hormone-dependant binding protein
Taglines A protein which binds and prolongs the half-life of the IGFs
NCBI Gene ID #. 3486
NCBI Gene Symbol IBP3
Gene Source Human
Accession # P17936
Source E. coli
Activity (Specifications/test method) N/A
Biological activity The ED₅₀ as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is
Molecular Weight 28.8 kDa
Storage Temp. -20°C
Shipping Gel pack
Shelf Life 1 year
Appearance Lyophilized protein
Physical form description Sterile filtered and lyophilized from 20 mM HCl
Reconstitution Instructions Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description IGF-I, IGF-II, insulin-like growth factors -1 and -2 are important regulators of signaling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades.
Additional source Recombinants or rec. proteins
Short name IGF-BP3, recombinant
Technique Recombinant, IGF
Alternative name IGF-BP3, H. sapiens recombinant
Alternative technique rec