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ELISA s application
For quantitative measurement of human DTK in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
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Gene tested
human DTK
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ELISA standard curve
Concentration(pg/ml) + 0 + 125 + 250 + 500 + 1000 + 2000 + 4000 + 8000 O.D + 0.191 + 0.253 + 0.314 + 0.481 + 0.642 + 1.063 + 1.455 + 2.116
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ELISA kit detection limits
125pg/ml to 8000pg/ml
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ELISA s sensitivity
< 10pg/ml
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Target of this ELISA
Natural and recombinant human DTK
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);647;2355;5632;729;2578;6348 ELISA standard deviations 34.29;153.07;371.71;53.94;183.04;533.23 CV(%);5.3;6.5;6.6;7.4;7.1;8.4
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ELISA kit description
GENTAUR's human DTK ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for DTK was used as capure antibodies on the ELISA test plate. The internal standards (Expression system for standard: NSO, Immunogen sequence: A41-S428) and your samples are measured with a biotin conjugated polyclonal from goat specific for DTK will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human DTK in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human DTK antibodies;1 ST0000-10;Lyophilized recombinant human DTK standard;10ng/tube×2 AR1107;Biotinylated anti- human DTK antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
heparin or EDTA
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human DTK standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each human DTK standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
15-20
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More gene info
Tyrosine-protein kinase receptor TYRO3 is an enzyme that in humans is encoded by the TYRO3 gene. The gene is part of a 3-member transmembrane receptor kinase receptor family with a processed pseudogene distal on chromosome 15. The encoded protein is activated by the products of the growth arrest-specific gene 6 and protein S genes and is involved in controlling cell survival and proliferation, spermatogenesis, immunoregulation and phagocytosis. The encoded protein has also been identified as a cell entry factor for Ebola and Marburg viruses.
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Antigen
NSO, A41-S428
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Tyrosine-protein kinase receptor TYRO3
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Gene name
TYRO3 protein tyrosine kinase
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Alternative
Brt ; BYK ; DTK ; RSE ; SKY ; Tif ; tyro3 ; Q06418
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Gen protein identity
Q06418
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Entrez nr
7301
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
TYRO3
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Short name
DTK/TYRO3 PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Human, Humans
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Alternative name
H. sapiens DTK/TYRO3 protein tyrosine kinase PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
TYRO3 protein tyrosine kinase, BYK and Dtk and RSE and Sky and Tif, TYRO3 and IDBG-7206 and ENSG00000092445 and 7301, transferase activity, nuclei, Tyro3 and IDBG-199993 and ENSMUSG00000027298 and 22174, TYRO3 and IDBG-646302 and ENSBTAG00000001694 and 788224
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