Histone H3, phosphorylated (Ser10) (Biotin)

Products_type

Antibody
Products_short_name

[Histone H3]
Products_name_syn

[Anti -Histone H3, phosphorylated (Ser10) (Biotin)]
Other_names

[histone H3; histone H3]
Other_gene_names

[H3]
Clonality

Polyclonal
Reactivity

Human
Specificity

Recognizes and is speci c for phosphorylated histone H3 (~17kD). Species Crossreactivity: Human. Broad species crossreactivity expected.
Purity

Affinity Purified Purified by Protein A affinity chromatography.
Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide. Labeled with Biotin.
Storage_stability

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Tested_application

Immunocytochemistry (ICC)
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated