CXCL14/BRAK, human recombinant
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Catalog number
4278-1000
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Price
Please ask
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Size
1 mg
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Synonyms
Chemokine BRAK, MIP-2G, Small-inducible cytokine B16
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Alternative_names
C-X-C motif chemokine 14 (Chemokine BRAK) (MIP-2G) (Small-inducible cytokine B14)
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Description
Chemoattractant for monocytes and dendritic cells
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Purity
≥95%
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Biological activity
The ED50 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells was 1.0-10.0 ng/ml.
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Results
1.0-10.0 ng/ml
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Molecular Weight
~9.4 kDa
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Lyophilized from a 0.2 um filtered solution of 20 mM Tris HCl and 1 M NaCl, pH 8.5.
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Reconstitution Instructions
Reconstitute the lyophilized recombinant human CXCL14 in sterile 18MΩ-cm H2O not less than 100 ug/ml. This can further be diluted to other aqueous buffers.
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Background Information
Human Chemokine (C-X-C motif) ligand 14(hCXCL14),also known as breast and kidney-expressed chemokine (BRAK), MIP-2 gamma, kidney-expressed chemokine (KEC), and B cell and monocyte-activating chemokine (BMAC), is a CXC chemokine constitutively expressed in certain normal tissues but is reduced or absent from many established tumor cell lines and human cancers. CXCL14 is known to be a chemo-attractant for monocyte and dendritic cells. CXCL14 inhibits angiogenesis and exhibits antimicrobial activities. Mature human and mouse CXCL14 differ by only 2 residues.
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Amino acid sequence
Recombinant Human CXCL14 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 9.4 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Cys-Lys-Cys.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CXCL14
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Short name
CXCL14/BRAK, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
chemokine (C-X-C motif) ligand 14/BRAK, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
chemokine (C-X-C motif) ligand 14, BMAC and BRAK and KEC and KS1 and MIP-2g and MIP2G and NJAC and SCYB14, CXCL14 and IDBG-45673 and ENSG00000145824 and 9547, chemokine activity, Extracellular, Cxcl14 and IDBG-157150 and ENSMUSG00000021508 and 57266, CXCL14 and IDBG-645845 and ENSBTAG00000006694 and 511771
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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