Rabbit Anti-Human AAK1 (pSer637) Polyclonal: Biotin
-
Catalog numberSPC-901D-BI
-
PricePlease ask
-
Size100 µl
-
-
ImmunogenA phospho-specific peptide corresponding to residues surrounding Ser637 of human AAK1 (AA634-640)
-
Antibody s targetHuman AAK1 (pSer637)
-
Antibody s full descriptionRabbit Anti-Human AAK1 (pSer637) Polyclonal
-
Antibody s categoryPolyclonal Antibodies
-
Antibody s other nameAAK1_HUMAN Antibody, Adaptor associated kinase 1 Antibody, AP2 associated kinase 1 AAntibody, DKFZp686F03202 Antibody, DKFZp686K16132 Antibody
-
Verified applicationsWB, AM
-
Raised inRabbit
-
Antibody s reactivityHuman
-
Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
-
Antibody s purified fromPeptide Affinity Purified
-
Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
-
Antibody s concentrationSee included datasheet or contact our support service
-
Antibody s specificityDetects 93.578 kDa.
-
Storage recommendations-20°C
-
Shipping recommendationsBlue Ice or 4°C
-
Certificate of analysisA 1:250 dilution of SPC-901 was sufficient for detection of AAK1 (pSer637) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cellMembrane
-
Tissue specificityDetected in brain, heart and liver.
-
Scientific contextAAK1 is a serine/threonine kinase that is thought to coordinate the recruitment of AP-2 to receptors containing tyrosine-based internalization motifs by phosphorylating the µ2 subunit. There is strong evidence that this protein is the endogenous mu 2 kinase and plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.
-
BibliographyRefer to PubMed
-
Released date29/Aug/2016
-
NCBI numberNP_002813
-
Gene number22848
-
Protein numberQ2M2I8
-
PubMed numberRefer to PubMed
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
-
Representative figure linkNo Data Available
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilitIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
TestStressMark antibodies supplies antibodies that are for research of human proteins.
-
PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
ConjugationBiotinylated
-
GroupPolyclonals and antibodies
-
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin nameOryctolagus cuniculus
-
Gene target
-
Gene symbolAAK1
-
Short nameRabbit Anti- AAK1 (pSer637) Polyclonal:
-
Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
HostRabbit, Rabbits
-
IsotypeImmunoglobulin
-
LabelBiotin
-
SpeciesHuman, Humans
-
Alternative nameproduction species: rabbit Antibody toHuman AAK1 (pSer637) polyclonal: biotinilated
-
Alternative techniquepolyclonals, rabbit-anti, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameAP2 associated kinase 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2003-07-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNA protein coding host genes
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data