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Also known as
ANTHRAX
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Other names
pXO1-110; Protective antigen; pXO1-110; N/A; Anthrax toxins translocating protein; PA-83
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
pxo1_110; pagA; N/A; pag; PA; PA83; PA20; PA63
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Gene name synonims
pxo1_110; pagA; N/A; pag; PA; PA83; PA20; PA63
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Other gene names
pxo1_110; pagA; N/A; pag; PA; PA83; PA20; PA63
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2b
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Clone
BAP0102
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Host organism
Mouse (Mus musculus)
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Species reactivity
Bacterial; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
ANTHRAX This item recognises the Protective Antigen of the anthrax toxin. Bacillus anthracis produces a toxin composed of three proteins, the Protective Antigen (PA), the Edema Factor (EF) and the Lethal Factor (LF). The three proteins depend on each other for their toxic effect. Both EF and LF functions intracellularly but are too large to enter the cell through existing channels. The PA is responsible for transporting the EF and LF into cells. The 83kDa PA (PA83) binds to the cell surface where a 20 kDa N-terminal fragment (PA20) is cleaved off, exposing binding sites for LF and EF. The remaining 63 kDa portion (PA63) oligomerizes to form a heptamer pre-pore, which then associates with up to three molecules of EF and/or LF. The receptor complex is internalized within endocytotic vessels that are subsequently acidified, transforming the PA heptamer into a pore that releases the active enzymes EF (an adenylate cyclase) and LF (a protease targeting MAPKKs) into the cytosol. _x000D__x000D_This item does not cross-react with the Lethal Factor of Bacillus anthracis, Yersinia pestis, Francisella tularensis or Toxoplasma gondii. The antibody recognises both the 83kDa and 63kDa truncated form of PA._x000D_; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Purified (Purified IgG - liquid)
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Concentration
IgG concentration 1.0mg/ml
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Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applications
ELISA (EIA), Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Antigens are peptides or recombinant or native dependent on the production method.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
