Prothrombin Antibody
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Catalog numberA1114-30T
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PricePlease ask
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Size30 µg
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Antibody TargetF2
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Target Alternative NameCoagulation factor II
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Antibody TypePolyclonal
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Species ReactivitiesHuman
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Immunogen SequenceHuman Prothrombin (Cat. No. 7684-1)
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AccessionP00734
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Gene Id2147
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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FormulationIn PBS pH 7.2, 0.01 % BSA, 0.03 % ProClin® and 50 % glycerol
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PurificationAffinity purified rabbit IgG
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ApplicationWB
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Positive ControlWB: h Prothrombin, h serum
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Application And UsagesWB: 1-4 µg
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DescriptionProthrombin is a vitamin K-dependent plasma protein which is synthesized in the liver. Prior to secretion into plasma, prothrombin undergoes post-translational modification by a vitamin K-dependent carboxylase which converts ten specific glutamic acid residues to γ-carboxyglutamic acid (gla). Conversion to thrombin is a key step in the blood coagulation pathway and catalyzes the coagulation of fibrinogen. Clinically, cases of selective deficiency are rare, although, in cases of liver cirrhosis, prothrombin is decreased. During activation, prothrombin is cleaved at Arg271-Thr272 and at Arg320-Ser321 to a "pro" fragment (fragment 1.2) and thrombin, the latter of which is composed of two chains covalently linked by a disulfide bond. There is an additional thrombin feed-back cleavage at Arg284-Thr285 resulting in an additional 13 amino acids being removed from the mature thrombin “A” chain.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolF7
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Short nameProthrombin Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameProthrombin (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namecoagulation factor VII
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Synonyms gene name
- coagulation factor VII (serum prothrombin conversion accelerator)
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Synonyms name
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Gla domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data