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Clone
S51-82
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Immunogen
Fusion protein amino acids 1-708 (full length) of rat MMP9
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Antibody s full description
Mouse Anti-Rat MMP9 Monoclonal IgG2a Antibody, Clone: S51-82: ATTO 594
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
MMP-9 Antibody, CLG4B Antibody, 82kDa matrix metalloproteinase-9 Antibody, collagenease type 4 beta Antibody, GELB Antibody, Macrophage gelatinase Antibody, MANDP2 Antibody, Type V collagenase Antibody, 92 kDa gelatinase Antibody, 92 kDa type IV collagenase Antibody, Gelatinase B Antibody, MMP9 Antibody, Matrix metalloproteinase 9 Antibody
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Raised in
Mouse
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Antibody s target
MMP9
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Primary research fields
Cell Signaling, Cancer, Apoptosis, Neuroscience, cardiovascular System
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, IP, FCM
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detecs ~92kDa and ~82kDa (pro and active forms).
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-396 was sufficient for detection of MMP9 in 20 µg of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Extracellular Matrix
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
MMP9, otherwise known as matrix metalloproteinase 9, is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction and tissue remodeling, as well as in disease processes like arthritis and metastasis (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).
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Bibliography
1. Hirose Y., et al. (2008) Am J Hum Genet. 82(5): 1122-1129. 2. Coussens L.M., et al. (2002) Science 295: 2387-2391. 3. Sternlicht M.D., et al. (1999) Cell 98: 137-146. 4. Vu T.H., et al. (1998) Cell 93: 411-422. 5. Nagase H., et al. (1990) Biochemistry 29: 5783-5789.
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Released date
16-May-2012
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PubMed number
25816747
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Tested applications
Flow Cytometry
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Tested reactivity
Mouse
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NCBI number
NP_112317.1
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Gene number
81687
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Protein number
P50282
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MMP9 Monoclonal Antibody, Clone S51-82 (SMC-396). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody (SMC-396) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) MMP9 Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~92 kDa and ~82 kDa (pro and active) MMP9 protein using Mouse Anti-MMP9 Monoclonal Antibody, Clone S51-82 (SMC-396). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody (SMC-396) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~92 kDa and ~82 kDa (pro and active). Mouse Anti-MMP9 Antibody [S51-82] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse NIH 3T3 (Mouse Fibroblast cell line) (SMC-396) Mouse Anti-MMP9 Antibody [S51-82] used in Western Blot (WB) on Rat Brain (SMC-396)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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Gene target
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Gene symbol
IGHVIII-82, MMP9, SNORD111, RNU6-82P, NUFIP2, OR4H6P
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Short name
Mouse Anti- MMP9 Monoclonal IgG2a, Clone S51-82
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Technique
anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG2a, IgG2a
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Label
ATTO 594
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Species
Rat, Mouses, Rats
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Alternative name
Mouse Antibody toRat matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) monoclonal IgG2a, clonality S51-82
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Alternative technique
murine, antibodies
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Alternative to gene target
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), CLG4B and GELB and MANDP2 and MMP-9, MMP9 and IDBG-78722 and ENSG00000100985 and 4318, identical protein binding, Extracellular, Mmp9 and IDBG-212451 and ENSMUSG00000017737 and 17395, MMP9 and IDBG-642990 and ENSBTAG00000020676 and 282871
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Clone name
S51-82
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