M13, fd, F1 Bacteriophages, Filamentous (Biotin)

• Catalog number
  MBS601918
• Price
  Please ask
• Size
  5x0.25 mL

• Products_type
  Antibody
• Products_short_name
  [M13, fd, F1 Bacteriophages, Filamentous]
• Products_name_syn
  [Anti -M13, fd, F1 Bacteriophages, Filamentous (Biotin)]
• Clonality
  Monoclonal
• Clone
  [5F167]
• Reactivity
  E Coli
• Specificity
  Binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT.
• Purity
  Affinity Purified Purified by Protein A affinity chromatography.
• Form
  Supplied as a liquid in PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Labeled with Biotin.
• Storage_stability
  May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
• Tested_application
  ELISA (EL/EIA), Western Blot (WB)
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Gene
  M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.

• Gene target
  M13   Bacteriophages   Filamentous  
• Short name
  M13, fd, F1 Bacteriophages, Filamentous ( )
• Host
  Mouse
• Isotype
  IgG2b
• Label
  Biotin
• Alternative name
  M13, fd, F1 Bacteriophages, Filamentous (biotinilated)

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