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Products_type
Secondary Antibody
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Products_short_name
[IgG (H&L)]
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Products_name_syn
[Donkey anti-Sheep IgG (H&L) - Affinity Pure]
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Reactivity
Sheep. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin sheep serum proteins IgG from human or rabbit serum proteins from human or rabbit
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Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on sheep IgG, light chains on all sheep immunoglobulins
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Sheep IgG.
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Form
Lyophilized
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Concentration
1.0 mg/ml
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Storage_stability
Store freeze-dried powder at 2-8 degree C. Shelf Life: Store lyophilized material at 2-8 degree C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 degree C as a liquid.
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Tested_application
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
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Test
A high affinity purification column was use to purify anti- IgG (H L) - Pure, Conjugate, min x w/ rabbit IgG by MyBioSource by chromatographic size exclusion.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure anti- IgG (H L) - Pure, Conjugate, min x w/ rabbit IgG supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
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Latin name
Equus africanus asinus, Oryctolagus cuniculus
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Gene target
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Short name
anti- IgG (H L) - Pure, Conjugate, min x w/ rabbit IgG
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Technique
affinity, Rabbit, anti-, anti, Pure, IgG, antibody to, antibodies, IgGs, pure products
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Host
Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. Rabbits
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Isotype
IgG, Heavy & Light chains, IgG
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Label
HRP
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Species
Human, Donkeys, Humans, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
Donkey Antibody toSheep Immunoglobulin G (heavy + light chain) - protein Pure, horseradish peroxidase Conjugate, min x w/H. sapiens and production species: rabbit Immunoglobulin G
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Alternative technique
rabbit-anti, antibodies, purified, immunoglobulins
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