Caspase-9, human recombinant

• Catalog number
  1089-100
• Price
  Please ask
• Size
  100 units

• Synonyms
  Caspase-9
• Alternative_names
  Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6, LEHDase
• Description
  An aspartic acid protease involved in apoptosis
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  > 400 units/mg
• Unit Definition
  One unit of the recombinant caspase-9 is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
• Storage Temp
  -70°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Semi Dry powder
• Reconstitution Instructions
  Reconstitute to 1 unit per ul in PBS containing 15% glycerol.
• Background Information
  Caspase-9 is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-9 also exists in cells as an inactive proenzyme. During the initiation of apoptosis procaspase-9 is processed at aspartate residues to form active caspase-9. As one of the initiator caspases, active caspase-9 functions to trigger activation of downstream effector caspases, leading to disassembly of cell structures. The recombinant active human caspase-9 was expressed in E. coli. The active caspase-9 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-LEHD-pNA (Cat. #1076-200) or Ac-LEHD-AFC (1075-200).
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Caspase-9  
• Gene symbol
  CARD9, CASP9, CARD19
• Short name
  Caspase-9, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  caspase-9, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:16391
• Gene
  CARD9
• Long gene name
  caspase recruitment domain family member 9
• Synonyms gene name
  • caspase recruitment domain family, member 9
• GenBank acession
  • AF311287
• Locus
  9q34.3
• Discovery year
  2001-08-13
• Entrez gene record
  64170
• Pubmed identfication
  • 11053425
• RefSeq identity
  • NM_052813
• Classification
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000020925
• Locus Specific Databases
  • LRG_178

Gene info

• Identity
  HGNC:1511
• Gene
  CASP9
• Long gene name
  caspase 9
• Synonyms gene name
  • caspase 9, apoptosis-related cysteine protease
  • caspase 9, apoptosis-related cysteine peptidase
• Synonyms
  • MCH6
  • ICE-LAP6
  • APAF-3
  • PPP1R56
• Synonyms name
  • protein phosphatase 1, regulatory subunit 56
• GenBank acession
  • U60521
• Locus
  1p36.21
• Discovery year
  1996-11-11
• Entrez gene record
  842
• Pubmed identfication
  • 8663294
  • 9390557
• RefSeq identity
  • NM_032996
• Classification
  • Caspases
  • Apoptosome
  • Caspase recruitment domain containing
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000002256

Gene info

• Identity
  HGNC:28148
• Gene
  CARD19
• Long gene name
  caspase recruitment domain family member 19
• Synonyms gene
  • C9orf89
• Synonyms gene name
  • chromosome 9 open reading frame 89
  • caspase recruitment domain family, member 19
• Synonyms
  • MGC11115
  • bA370F5.1
  • BinCARD
• Synonyms name
  • Bcl10-interacting protein with CARD
• GenBank acession
  • AK057716
• Locus
  9q22.31
• Discovery year
  2004-02-19
• Entrez gene record
  84270
• Pubmed identfication
  • 15637807
• RefSeq identity
  • NM_032310
• Classification
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000020243

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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