XRCC4 Antibody
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Catalog numberR32215
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenXRCC4
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC-P
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Recommended dilutionsWestern blot: 0.1-0.5ug/ml,IHC (Paraffin): 0.5-1ug/ml
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NotesOptimal dilution of the XRCC4 antibody should be determined by the researcher.
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Intented useThis XRCC4 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ13426
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PurityAntigen affinity
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DescriptionX-ray repair cross-complementing protein 4 is a protein that in humans is encoded by the XRCC4 gene. In addition to humans, the XRCC4 protein is also expressed in many other metazoans, fungi and in plants. The X-ray repair cross-complementing protein 4 is one of several coreproteins involved in the non-homologous end joining (NHEJ) pathway to repair DNA double strand breaks (DSBs). Since XRCC4 is the key protein that enables interaction of LigIV to damaged DNA and therefore ligation of the ends, mutations in the XRCC4 gene were found to cause embryonic lethality in mice and developmental inhibition and immunodeficiency in humans. Furthermore, certain mutations in the XRCC4 gene are associated with an increased risk of cancer.
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ImmunogenAmino acids ESEISQEADDMAMEKGKYVGELRKALL of human XRCC4 were used as the immunogen for the XRCC4 antibody.
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StorageAfter reconstitution, the XRCC4 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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LocalizationNuclear
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolXRCC4
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Short nameAnti-XRCC4
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to XRCC4
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Alternative techniqueantibodies
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Alternative to gene targetX-ray repair complementing defective repair in Chinese hamster cells 4, XRCC4 and IDBG-32219 and ENSG00000152422 and 7518, protein C-terminus binding, nuclei, Xrcc4 and IDBG-171005 and ENSMUSG00000021615 and 108138, XRCC4 and IDBG-646520 and ENSBTAG00000024275 and 613590
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Gene info
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Identity
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Gene
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Long gene nameX-ray repair cross complementing 4
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Synonyms gene name
- X-ray repair complementing defective repair in Chinese hamster cells 4
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Synonyms name
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GenBank acession
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Locus
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Discovery year1990-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data