Streptavidin-FITC

Keep at

-20° C
Reagent list
Assay performance

TACS for in situ detection of apoptosis in a TUNEL test with frozen, paraffin embedded tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA with enzymes and permeabilization reagents, labeling and detection reagents, stop buffers, counterstain and TACS-Nuclease for in situ labeling.
More advice

Streptavidin HRP and and TACS Nucleases for positive controls are supplied for immunohistochemistry with HRP an DAB with incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA for apoptosis detection.
Other trevi products
Properties

This Trevigen Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Trevigen FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody

Name

Cytophotometry
Concept

Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data