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Alternative name 1
CD5, Clone: DK23, FITC + CD19l, Clone: HD37, RPE, Mab anti-Human, dual label
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Alternative name 2
CD5, Clone: DK23, FITC + CD19l, Clone: HD37, RPE, Mab antibody-Human, dual label
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Alternative name 3
CD5, Clone: DK23, FITC + CD19l, Clone: HD37, RPE, Mab antibody-Homo sapiens, dual label
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Alternative name 4
anti-CD5 Clone: DK23 + CD19l Clone: HD37 dual label
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Alternative name 5
CD5 Clone: DK23 + CD19l Clone: HD37 dual label
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
The duality of this product lies in it's dual specificity to the gene, pH or protein. Mainly in phosphorylation by specific phosphatases. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Conjugation
Anti-FITC Antibody
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Additional description
Brady printing labels are printed by a label printer for labeling with 6,5 m long cassettes.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
RPE, CD5
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Short name
CD5, Clone: DK23, FITC + CD19l, Clone: HD37, RPE, Mouse Monoclonal antibody- ,
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Technique
Mouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Label
FITC
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Species
Human, Humans, Mouses
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Alternative name
CD5 molecule, clonality: DK23, fluorecein + CD19l, clonality: HD37, RPE, Mouse monoclonal (antibody to-)-H. sapiens, dual label
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Alternative technique
antibodies, murine, fluorescine
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Alternative to gene target
CD5 molecule, LEU1 and T1, CD5 and IDBG-49730 and ENSG00000110448 and 921, protein binding, Cell surfaces, Cd5 and IDBG-144910 and ENSMUSG00000024669 and 12507, CD5 and IDBG-643103 and ENSBTAG00000013730 and 280745
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