Crkl(Tyr251) Antibody, Cy3 Conjugated
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Catalog number
bs-3738R-Cy3
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Price
Please ask
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Size
0.1ml
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Long name
Crkl(Tyr251) Polyclonal Antibody, Cy3 Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Cy3
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
CrklTyr251
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Specificity
This is a highly specific antibody against CrklTyr251.
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Modification
Phosphorylation
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Modification site
Tyr251
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
230-270/303
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Subcellular location
Intracellular
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Source
KLH conjugated synthetic phosphopeptide derived from human Crkl around the phosphorylation site of Tyr251 [CA(p-Y)D]
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Gene ID number
1399
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Swiss Prot
P46109
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Crkl is a 34kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
512,550nm/570,615nm
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Synonyms
Crk-like protein; CRKL
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-CrklTyr251 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
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Additional conjugation
cy3 conjugation kit
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French translation
anticorps
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Gene target
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Short name
Anti-CrklTyr251
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Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
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Isotype
Immunoglobulin G (IgG)
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Label
Cy3
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Alternative name
Anti-CrklTyr251 PAb Cy3
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Alternative technique
antibodies
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Alternative to gene target
v-crk sarcoma virus CT10 oncogene homolog (avian)-like, CRKL and IDBG-1833 and ENSG00000099942 and 1399, poly(A) RNA binding, Cytoplasm, Crkl and IDBG-138913 and ENSMUSG00000006134 and 12929, CRKL and IDBG-637664 and ENSBTAG00000045548 and
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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