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Clone
25F2.D10
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Immunogen
Mouse Aha1
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Antibody s full description
Rat Anti-Mouse AHA1 Monoclonal IgG2a Kappa Antibody, Clone: 25F2.D10: ATTO 680
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody
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Raised in
Rat
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Antibody s target
AHA1
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Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, IP, ELISA
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP ( 1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
0.02M potassium phosphate, 0.15M sodium chloride, pH7.2
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Antibody s concentration
1.59 mg/ml
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Antibody s specificity
Detects ~38kDa. Can run up to 45kDa on SDS Page.
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Storage recommendations
4ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-173 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm , Endoplasmic Reticulum
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
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Bibliography
1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
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Released date
1-Jan-2010
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_666148.1
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Gene number
217737
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Protein number
Q8BK64
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (SMC-173). Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (SMC-173) at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green). Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (SMC-173). Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (SMC-173) at 1:1000. Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (SMC-173). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (SMC-173) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle. Western Blot analysis of Human Cell lysates showing detection of Aha1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (SMC-173). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (SMC-173) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Rat Anti-Aha1 Antibody [25F2.D10] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa cells (SMC-173) Rat Anti-Aha1 Antibody [25F2.D10] used in Immunoprecipitation (IP) on Human HeLa cells (SMC-173) Rat Anti-Aha1 Antibody [25F2.D10] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-173) Rat Anti-Aha1 Antibody [25F2.D10] used in Western Blot (WB) on Human Cell lysates (SMC-173)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
