Histone H1.2 polyclonal antibody

Alternative Name

HIST1H1C, H1F2, H1 histone family member 2, H1.a, H12, H1F2, H1s-1, HIST1H1C, Histone 1 H1c, Histone cluster 1 H1c, Histone H1.2, Histone H1c, Histone H1d, Histone H1s-1, MGC3992,
Immunogen

Recombinant protein
Swiss Prot

P16403(Human) P15864(Mouse) P15865(Rat)
Reactivity

Human, Mouse, Rat
Applications

WB ICC IF IHC ChIP
Application_all

WB:1:500-1:2,000 ICC:1:50-1:100 IHC:1:50-1:200
Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification Purity

ProA affinity purified
Storage Stability

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity

Histone H1.2 polyclonal antibody detects endogenous levels of Histone H1.2 protein.
Usage

Western blot analysis of Histone H1.2 on different cell lysates using anti-Histone H1.2 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: MCF-7
BiowMW

29 kDa
Note

For research use only, not for use in diagnostic procedure.
Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation

anticorps

Gene target

Histone
Gene symbol

H1-2, H3P26, H1-3
Short name

Histone H1 2 polyclonal antibody
Technique

Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Host

Rabbit
Alternative name

Histone H1.2 polyclonal (antibody to-)
Alternative technique

polyclonals, antibodies

Identity

HGNC:4716
Gene

H1-2
Long gene name

H1.2 linker histone, cluster member
Synonyms gene
- H1F2
- HIST1H1C
Synonyms gene name
- H1 histone family, member 2
- histone 1, H1c
- histone cluster 1, H1c
- histone cluster 1 H1 family member c
Synonyms
- H1.2
- H1s-1
- H1c
GenBank acession
- X57129
Locus

6p22.2
Discovery year

2001-06-22
Entrez gene record

3006
Pubmed identfication
- 2759094
- 12408966
RefSeq identity
- NM_005319
Classification
- H1 histones
VEGA ID

OTTHUMG00000016140

Identity

HGNC:4770
Gene

H3P26
Long gene name

H3 histone pseudogene 26
Synonyms gene
- H3FEP
- HIST1H1PS2
Synonyms gene name
- H3 histone family, member E, pseudogene
- histone 1, H1, pseudogene 2
- histone cluster 1 H1 pseudogene 2
Synonyms
- pH3/e
GenBank acession
- X67080
Locus

6p22.2
Discovery year

1998-10-05
Entrez gene record

10338
Pubmed identfication
- 9031620
RefSeq identity
- NG_029342
VEGA ID

OTTHUMG00000036301

Identity

HGNC:4717
Gene

H1-3
Long gene name

H1.3 linker histone, cluster member
Synonyms gene
- H1F3
- HIST1H1D
Synonyms gene name
- H1 histone family, member 3
- histone 1, H1d
- histone cluster 1, H1d
- histone cluster 1 H1 family member d
Synonyms
- H1.3
- H1d
- H1s-2
GenBank acession
- M60747
Locus

6p22.2
Discovery year

2001-06-22
Entrez gene record

3007
Pubmed identfication
- 1916825
- 12408966
RefSeq identity
- NM_005320
Classification
- H1 histones
VEGA ID

OTTHUMG00000014432

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data