YAP1 Antibody
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Catalog numberF49693-0.08ML
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PricePlease ask
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Size0.08 ml
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CategoryAntibody
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ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
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FormPurified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenYAP1
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsFACS, WB, ELISA
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Recommended dilutionsWestern blot: 1:1000,Flow Cytometry: 1:10-1:50
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NotesTitration of the YAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis YAP antibodyis to be used only for research purposes and not for diagnostics..
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UniprotP46937
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PurityPurified
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DescriptionYAP is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. [UniProt]
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ImmunogenA portion of amino acids 420-446 from human YAP1 was used as the immunogen for this YAP antibody.
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StorageAliquot the YAP antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolYAP1
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Short nameAnti-YAP1
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to YAP1
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameYes1 associated transcriptional regulator
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Synonyms gene name
- Yes-associated protein 1, 65kDa
- Yes associated protein 1
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Synonyms
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Locus
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Discovery year2001-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data