Target of this antibody:
Alternative names of antibody target:
Anti-sheep IgG, HRP-Linked Antibody, HRP linked secondary antibody
Rabbit Polyclonal Secondary Antibody to Sheep IgG (H&L) HRP.
This antibody reacts with:
Sheep IgG (H&L)
Formulation of this antibody:
In 0.01M Sodium phosphate, 0.25 M NaCl, 1% BSA, 50% Glycerol, pH 7.6 and 0.05% Sodium Azide.
This antibody has been succesfully tested for use in following methods:
ELISA, WB, IH, IC.
ELISA: 1:5000 – 1:100,000, WB: 1:5000 – 1:100,000, IH: 1:500 – 1:5000, IC: 1:500 – 1:5000.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Heavy & Light chains,IgG
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.