Anti-Phospho-ERK1/2 (Thr202/Tyr204) Antibody
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Catalog numberA1589-100
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PricePlease ask
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Size100 µg
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Antibody TargetMitogen-activated protein kinase 3
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Target Alternative NameMitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERK-1, Extracellular signal-regulated kinase 1, ERK-1
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Antibody TypePolyclonal
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Species ReactivitiesHuman, Mouse, Rat
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Immunogen SequenceA synthesized peptide derived from human ERK1/2 around the phosphorylation site of Threonine 202/Tyrosine 204
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AccessionP27361
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Gene Id5595
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AppearanceColorless liquid
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FormLiquid
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FormulationIn phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
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PurificationAffinity purified
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ApplicationWB, IHC, IF
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Positive ControlWB: HeLa cell lysate, IHC: human small instestinal carcinoma, IF: lovo cells
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Application And UsagesWB; 1:500 – 1:2000, IHC; 1:50- 1:200, IF; 1-200
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DescriptionSerine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedVerified
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Immunofluorescence VerifiedVerified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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Additional descriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMAPK3
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Short nameAnti-Phospho-ERK1/2 (Thr202/Tyr204) Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibody tophosphorilated-ERK1/2 (Thr202/Tyr204) (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namemitogen-activated protein kinase 3
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Mitogen-activated protein kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data