Clathrin heavy chain monoclonal antibody

  • Catalog number
    11-526-C025
  • Price
    Please ask
  • Size
    25ug
  • Long name
    Mouse Monoclonal Antibody against Human Clathrin heavy chain, Clone: BF-06
  • Antigen
    Clathrin heavy chain
  • Species reactivity
    Human (Homo sapiens), Porcine, Mouse (Mus musculus), Rat, Bovine, Other not determined
  • Negative species reactivity
    at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    BF-06
  • Applications
    FC, IP, WB, ICC, ELISA
  • Format conjugation
    purified; unconjugated
  • Specificity
    The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis. _x000D_
  • Research area
    Endocytosis (Veterinary), Endocytosis (Rodent), Endocytosis (Human)
  • Concentration
    1 mg/ml
  • Storage buffer
    Stabilising Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
  • Shipping and storage
    Mouse Monoclonal Antibody against Human Clathrin heavy chain, Clone: BF-06 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
  • Background
    Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by precipitation and chromatography
  • References
    *Macurek L, Zikova M, Draberova E, Draber P: Monoclonal antibody BF-06 against the heavy chain of clathrin. Folia Biol (Praha). 2003;49(6):238-40._x000D_
  • Properties
    If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • French translation
    anticorps
  • Gene target
  • Short name
    Anti-Clathrin heavy chain Mab
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Host
    Mouse (Mus musculus)
  • Isotype
    IgM
  • Alternative name
    Mouse Monoclonal to Clathrin heavy chain
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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