-
Long name
Mouse Monoclonal Antibody against Human Clathrin heavy chain, Clone: BF-06
-
Antigen
Clathrin heavy chain
-
Species reactivity
Human (Homo sapiens), Porcine, Mouse (Mus musculus), Rat, Bovine, Other not determined
-
Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
-
Clonality
Monoclonal Antibody (Mab)
-
Clone
BF-06
-
Applications
FC, IP, WB, ICC, ELISA
-
Format conjugation
purified; unconjugated
-
Specificity
The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis. _x000D_
-
Research area
Endocytosis (Veterinary), Endocytosis (Rodent), Endocytosis (Human)
-
Concentration
1 mg/ml
-
Storage buffer
Stabilising Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
-
Shipping and storage
Mouse Monoclonal Antibody against Human Clathrin heavy chain, Clone: BF-06 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
-
Background
Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.
-
Purity
> 95% (by SDS-PAGE)
-
Purification method
Purified by precipitation and chromatography
-
-
References
*Macurek L, Zikova M, Draberova E, Draber P: Monoclonal antibody BF-06 against the heavy chain of clathrin. Folia Biol (Praha). 2003;49(6):238-40._x000D_
-
Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
French translation
anticorps