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Synonyms
Apolipoprotein A-I
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Alternative_names
APOA1, MGC117399, Apoa1, Alp-1, Apoa-1, Brp-14, Ltw-1, Lvtw-1, apolipoprotein, apolipoproteins, MGC102525, Sep-1, Sep-2, Sep2, rat ApoA-1, ApoA-1, ApoA1, Apo A1, r-ApoA-1, rr-ApoA-1, recombinant rat ApoA-1, ApoA1, recombinant ApoA-1, ApoA1, Apolipoprotein A-I, Apolipoprotein AI, Apolipoprotein
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Description
A protein promoting cholesterol efflux from tissues to the liver
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Purity
≥98%
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Activity Specifications test method
Test in process
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Molecular Weight
27.4 kDa
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Storage Temp
-20°C
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Shipping
Blue ice
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Sterile filtered and lyophilized with no additives
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Reconstitution Instructions
Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.
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Background Information
ApoA-I is a 29.0 kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle. ApoA-I, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This apoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is though to inhibit atherogenesis. For this reason HDL is also known as the “good cholesterol.” The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I (called apoA-I Milano). The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic Vascular diseases. Recombinant rat ApoA-I is a 27.4 kDa protein of 235 amino acid residues.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Additional source
Recombinants or rec. proteins
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Group
recombinants
