CytoSelect LDH Cytotoxicity Assay Kit

• Catalog number
  CBA-241
• Price
  Please ask
• Size
  960 reactions

• Type of the product
  Set of reagents
• Product description
  This CytoSelect LDH Cytotoxicity Assay Set of reagents provides a colorimetric format for measuring and monitoring cell cytotoxicity.  The Set of reagents contains sufficient reagents for the evaluation of 960 reactions in 96-well plates.  Cells can be plated and then treated with compounds or agents that affect cell viability.  Upon cell death, lactate dehydrogenase (LDH), a soluble enzyme found in the cytoplasm, is released into the growth media.  The growth media is then transferred to another plate and the released LDH is then detected with cytotoxicity reagent.  In the presence of lactate substrate (included in the LDH Cytotoxicity Reagent) LDH converts lactate to pyruvate and generates nicotinamide adenine dinucleotide (NADH).   The WST-1 molecule, also present in the LDH Cytotoxicity Reagent, is converted from WST-1 to the orange formazan form.  An increase in cell cytotoxicity is accompanied by increased LDH release and increased colorimetric signal.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues, depending on LDH expression levels.  The LDH Cytotoxicity Reagent can be used to detect cytotoxicity in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.
• Shipping conditions
  The product CytoSelect LDH Cytotoxicity Assay Kit must be shipped on blue ice packages to retain the activity and stability of the reagents.
• Storage conditions
  After the delivery of this product, our specialists from Gentaur/Genprice recommend you to prepare aliquots to minimize the risk of repeated cycles of freezing and thawing. The standard suspension should be kept at minus twenty degrees Celsius but the rest of the reagents can be stored at four degrees Celsius.
• Tips
  This product is manufactured for research purposes only and it is not intended to be used in clinical diagnostic procedures.
• Test
  Cellular biology labs supplies other types of Assays as 1.

• Gene target
  CytoSelect   LDH   Cytotoxicity   Kit  
• Short name
  CytoSelect LDH Cytotoxicity Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  CytoSelect LDH Cytotoxicity test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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