Rabbit Polyclonal Antibody Anti Caspase-3 active
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Catalog number
CAS3PU
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Price
Please ask
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Size
0,1 ml
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Clone
Rabbit Polyclonal
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Conjugated
PURE
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Species reactivity
Human
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Immunogen
synthetic peptide (KLH-conjugated) corresponding to amino terminal residues adjacent to Asp175 of human caspase-3.
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Tested application
Western Blot (1:3000)
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Long description
The Rabbit Polyclonal Antibody Anti Caspase-3 active is recommended to detect specifically in Western Blot technique caspase 3 (CPP-32, Apoptain, Yama, SCA-1)
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Alternative names
CASP3, CPP32
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Storage buffer
PBS, 0,09% sodium Azide, may contain carrier protein-stabilizer
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Gene number
836
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UniProt antigen number
P42574
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Molecular weight
17 kDa
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Shipping conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Technical datasheet
Please contact us to receive the latest datasheet, the certificate of analysis or the MSDS
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Regulation status
This antibody is intended for Research Use Only.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by Immunostep. Rabbit antibodies are very stable and can be stored for several days at room temperature. Immunostep adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Rabbit Polyclonal Antibody Anti Caspase-3 active
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Technique
Polyclonal, Antibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Polyclonal
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Alternative name
production species: rabbit polyclonal (antibody to-) antibody to caspase-3 functionnal
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Alternative technique
polyclonals, antibodies, rabbit-anti
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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