Akt Negative Control Cell Lysate

Description

Uninduced Jurkat cell extracts useful as a control in western blotting or activity assays.. Jurkat cells growing in DMEM supplemented with 10% FBS were collected and washed in ice-cold PBS. Cells were then lyzed in three volume of Kinase Extraction Buffer (70002-N80M in K435-40), keep on ice for 5 min, centrifuge at top speed for 15 min. Collect the cell lysate. Extract the pellet again with one volume Kinase Extraction Buffer. Combine the cell lysate together, determine protein concentration. The cell lysate can be used as a negative control for Akt activity assays, in conjunction with the use of Akt Activity Assay Kit (K435-40). We recommend using 100 µg per assay.
Product Highlights

• CONCENTRATION- 4 mg/ml
Storage Temp

-70°C
Shipping

dry ice
Shelf Life

6 months
Datasheet
Notes

For research use only. Not to be used for human or animal treatment or consumption.
Additional description

For cells, cell lines and tissues in culture till half confluency. Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.

Name

Negative Staining
Concept

Scope note: The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
Tree numbers
- E01.370.225.500.620.670.520
- E01.370.225.750.600.670.520
- E05.200.500.620.670.520
- E05.200.750.600.670.520
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data