Phospho-ERK1/2 (Thr202/Tyr204) Translocation Assay Kit (Cell-Based)
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Catalog number
K696-50
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Price
Please ask
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Size
50 assays
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Description
A cell-based assay kit that detect the nuclear translocation of activated ERK1/2 in fixed mammalian cells.
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Summary
• Detection method- fluorescence microscope capable of measuring EX at 570 nm and equipped with UV filter for DAPI • Species reactivity- Human, mouse, rat • Application- Detection of nuclear translocation of phosphorylated-ERK1/2 in mammalian cells
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Detection Method
fluorescence microscope capable of measuring EX at 570 nm and equipped with UV filter for DAPI
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Species Reactivity
Human, mouse, rat
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Applications
Detection of nuclear translocation of phospo-ERK1 and phospo-ERK2 in mammalian cells
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Sample Type
Adherent or suspension mammalian cell cultures
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Features Benefits
• Detection of subcellular localization of activated ERK1/2 in mammalian cells • Screening and characterizing effectors of ERK1/2 kinases • Studying of cell signaling, cell division and cell proliferation mechanism
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Mitogen-activated protein kinases (MAPKs) are proline-directed serine and threonine protein kinases that regulate numerous physiological cell responses including: embryogenesis, cell differentiation, proliferation, migration, apoptosis and death. Extracellular signal-regulated kinases (ERKs) 1 and 2 (ERK1/2), also known as p44 MAPK and p42 MAPK respectively, belong to one of the five major groups of MAPKs. Closely-related ERK1/2 isoforms are uniquely activated by several extracellular signals including growth factors, cytokines, hormones, and neuro-transmitters. Activation of ERK1/2 by the upstream kinases MEK1 and MEK2 occurs via dual phosphorylation on specific threonine (Thr202) and tyrosine (Tyr204) residues on the T*EY* motif. MEK1 and MEK2 are activated through receptors (tyrosine kinases or integrins) via pathways involving adaptor proteins, guanine nucleotide exchange factors, small GTP binding proteins, and MAPKKs. Activated ERK1/2 phosphorylates both, cytosolic (SOS, MNK1/2, RSKs) and nuclear targets. In the nucleus, it affects gene expression and DNA replication by the phosphorylation of MSK 1 and 2 and the transcription factors Elk-1, Sap1, and Sap2. In cultured cells, growth factors or mitogens induce rapid and transient translocation of activated ERK1/2 to nucleus. Different cell lines exhibit various duration, magnitude, and subcellular localization of activated/phosphorylated ERK1/2. The response of the protein may differ even within the same cell line depending on the dose and cell density. BioVision’s Phospho-ERK1/2 (Thr202/Tyr204) Translocation Assay Kit provides a simple and complete assay in a ready-to-use format to visualize the translocation of activated ERK1/2 between cytoplasmic and nuclear compartments in mammalian cells.
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Test
Biovision supplies other types of Assays as 1.
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Additional description
For cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbol
MAPK3
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Short name
Phospho-ERK1/2 (Thr202/Tyr204) Translocation Assay Kit ( -Based)
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Technique
Assay, assays
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Host
Assay
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Alternative name
phosphorilated-ERK1/2 (Thr202/Tyr204) Translocation test reagent (cellular-Based)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell
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Gene info
Product images
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