TACS for in situ detection of apoptosis in a TUNEL test with frozen, paraffin embedded tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA with enzymes and permeabilization reagents, labeling and detection reagents, stop buffers, counterstain and TACS-Nuclease for in situ labeling.
Streptavidin HRP and and TACS Nucleases for positive controls are supplied for immunohistochemistry with HRP an DAB with incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA for apoptosis detection.
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.