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Category
Primary Antibodies
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Long description
Human acid sphingomyelinase (sphingomyelin phosphodiesterase, ASM) is the lysosomal enzyme responsible for the hydrolysis of sphingomyelin to ceramide and phosphocholine. Converts sphingomyelin to ceramide. aSM also has phospholipase C activities toward 1,2-diacylglycerol-phosphocholine and 1,2-diacylglycerol-phosphoglycerol. The enzyme is a membrane-associated glycoprotein with a pH optimum of about 4.5 and a subunit molecular mass of about 72 kDa. In addition AtoS M, two other sphingomyelinases have been identified in man, a Mg2+- dependent neutral sphingomyelinase found primarily in brain and a Zn2+-dependent acid sphingomyelinase found primarily in serum. Although it is likely that the acid and neutral sphingomyelinases are coded by different genes, the molecular genetic relationship of these three biochemically distinct sphingomyelinases has not been determined. Understanding the role of these sphingomyelinases in the hydrolysis of sphingomyelin to ceramide will be an important step in the understanding of ceramide as it is further hydrolyzed to sphingosine, a neutral phospholipid which has been implicated in the regulation of protein kinase C-mediated signal transduction. Inherited deficiencies of ASM have been reported in man, deficient ASM activity results in the two major subtypes of Niemann-Pick disease (NPD).
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Antibody come from
Synthetic peptide derived from human phosphatidic acid phosphatase type 2 domain containing 2 (PPAPDC2)
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Other description
Provided as ligand affinity purified antibody in phosphate buffered saline with 0.1% BSA and 0.08% sodium azide. Ammonium Sulfate Precipitation
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Acid Sphingomyelinase Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, ELISA
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Antibody s suited for
Antibody can be used for Western blotting (1:400-1:800 dilution). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. 'IDENTIFICATION AND FUNCTIONAL CHARACTERIZATION OF A_x000B_PRESQUALENE DIPHOSPHATE PHOSPHATASE.' Koichi Fukunaga, Makoto Arita, Minoru Takahashi, Andrew J. Morris, Michael Pfeffer and Bruce D. Levy JBC Papers in Press. Published on February 7, 2006 as Manuscript M512970200_x000B__x000B_2. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., , Marra M.A.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
