Anti-free PSA Antibody (1B4A5)

• Catalog number
  A1352-1000
• Price
  Please ask
• Size
  1 mg

• Antibody Target
  Prostate-specific antigen
• Target Alternative Name
  Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, APS
• Antibody Type
  Monoclonal
• Clone
  1B4A5
• Species Reactivities
  Human
• Immunogen Sequence
  Free PSA protein purified from human seminal fluid
• Accession
  P07288
• Gene Id
  354
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS buffer, pH 7.4, containing 0.02% sodium azide
• Purification
  Protein A purified
• Application
  ELISA
• Positive Control
  ELISA
• Application And Usages
  ELISA Capture: 1-10 µg/ml, ELISA Detection: 0.05-0.2 µg/ml
• Description
  Prostate-specific antigen (PSA) is produced by cells of the prostate gland. The PSA level increases in the blood of men who have prostate cancer, benign prostatic hyperplasia, or infection or inflammation of the prostate. It exists in the blood in two forms of free PSA and total PSA (PSA attached to a protein molecule). Free PSA increases more in benign prostatic hypertrophy while total PSA is associated with cancer. When Prostate cancer occurs, PSA often ranges from 2.5 to 10 ng/ml. The free PSA and total PSA are tested simultaneously to identify what kind of prostate problem a man has. Free PSA Antibody is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with free PSA protein purified from human seminal fluid
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Not Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  free   PSA   1B4A5  
• Gene symbol
  KLK3, NPEPPS, PSAT1
• Short name
  Anti-free PSA Antibody (1B4A5)
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Mouse IgG1, κ
• Alternative name
  Antibody tofree PSA (antibody to-) (1B4A5)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6364
• Gene
  KLK3
• Long gene name
  kallikrein related peptidase 3
• Synonyms gene
  • APS
• Synonyms gene name
  • kallikrein 3, (prostate specific antigen)
• Synonyms
  • PSA
• GenBank acession
  • X14810
• Locus
  19q13.33
• Discovery year
  2001-06-22
• Entrez gene record
  354
• Pubmed identfication
  • 2456523
  • 2436946
  • 16800724
  • 16800723
• RefSeq identity
  • NM_145864
• Classification
  • Kallikreins
• VEGA ID
  OTTHUMG00000182867

Gene info

• Identity
  HGNC:7900
• Gene
  NPEPPS
• Long gene name
  aminopeptidase puromycin sensitive
• Synonyms
  • PSA
  • MP100
• Synonyms name
  • puromycin-sensitive aminopeptidase
  • metalloproteinase MP100
• GenBank acession
  • Y07701
• Locus
  17q21.32
• Discovery year
  1999-12-09
• Entrez gene record
  9520
• Pubmed identfication
  • 9048733
  • 10329370
• RefSeq identity
  • NM_006310
• Classification
  • M1 metallopeptidases
  • Aminopeptidases
• VEGA ID
  OTTHUMG00000165471

Gene info

• Identity
  HGNC:19129
• Gene
  PSAT1
• Long gene name
  phosphoserine aminotransferase 1
• Synonyms
  • PSA
  • PSAT
• Synonyms name
  • phosphoserine transaminase
• GenBank acession
  • BC004863
• Locus
  9q21.2
• Discovery year
  2003-05-19
• Entrez gene record
  29968
• Pubmed identfication
  • 12633500
  • 3651428
• RefSeq identity
  • NM_021154
• VEGA ID
  OTTHUMG00000020066

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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