Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 3:1 Mixture), Low glucose w/o L-Glutamine, Calcium chloride, HEPES buffer and Sodium bicarbonate

  • Catalog number
    AT228A-20LX2
  • Price
    Please ask
  • Size
    2X20L
  • Description
    Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 3:1 Mixture), is a White to light pink, homogenous powder Low glucose,w/o L-Glutamine, Calcium Chloride, HEPES buffer and Sodium bicarbonate,Produced by HiMedia Laboratories Private Limited
  • Similar products
  • Shelf Life
    Use before the expiry date
  • Storage conditions
    store between 2-8°C
  • Appearance
    White to light pink, homogenous powder
  • Solubility
    Soluble in water
  • Use
    used in laboratory chemicals, Manufacture of substances
  • Technical data
    Please contact us to request the technical data
  • Data sheet
  • Additional description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Group
    media
  • Gene target
    Dulbecco’s   Modified   Eagle   Nutrient   Mixture   F-12   Ham   DMEM   F12   Mixture   glucose   w/o   L-Glutamine   Calcium   chloride   Sodium   bicarbonate  
  • Gene symbol
    F12, HLA-F, SNAR-F, H3C11, IGHV1-69-2, KLRC4, PIRC55, PIRC54, PIRC53
  • Short name
    Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 3:1 Mixture), glucose w/o L-Glutamine, Calcium chloride, Sodium bicarbonate
  • Technique
    Medium, cell culture Media
  • Alternative name
    Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 3:1 Mixture), Low glucose w/o L-Glutamine, Calcium chloride, HEPES solution and Sodium bicarbonate
  • Alternative technique
    cellculture
  • Virus
    hhv
Gene info
Gene info
Gene info
  • Identity
    HGNC:34326
  • Gene
    SNAR-F
  • Long gene name
    small NF90 (ILF3) associated RNA F
  • Synonyms gene name
    • small nuclear ILF3/NF90-associated RNA F
    • small ILF3/NF90-associated RNA F
  • GenBank acession
  • Locus
    19q13.33
  • Discovery year
    2008-06-20
  • Entrez gene record
    100126781
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Small NF90 (ILF3) associated RNAs
Gene info
Gene info
Gene info
  • Identity
    HGNC:6377
  • Gene
    KLRC4
  • Long gene name
    killer cell lectin like receptor C4
  • Synonyms gene name
    • killer cell lectin-like receptor subfamily C, member 4
  • Synonyms
  • GenBank acession
  • Locus
    12p13.2
  • Discovery year
    1998-09-03
  • Entrez gene record
    8302
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Killer cell lectin like receptors
  • VEGA ID
    OTTHUMG00000168575
Gene info
  • Identity
    HGNC:37559
  • Gene
    PIRC55
  • Long gene name
    piwi-interacting RNA cluster 55
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
    100313792
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
    HGNC:37558
  • Gene
    PIRC54
  • Long gene name
    piwi-interacting RNA cluster 54
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
    100313815
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
    HGNC:37557
  • Gene
    PIRC53
  • Long gene name
    piwi-interacting RNA cluster 53
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
    100313861
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
MeSH Data
  • Name
    In Vitro Techniques
  • Concept
    Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
  • Tree numbers
    • E05.481
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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