Hi-Bind™ Protein A-Agarose

  • Catalog number
    6520-5
  • Price
    Please ask
  • Size
    5 ml
  • Description
    Reusable Protein A conjugated hi bind agarose beads with ≥36 mg/ml binding capacity & 2.89 ml/min flow rate for antibody purification from multiple sources. Hi-BindTM Protein A-Agarose beads are specially prepared for high IgG binding by covalently coupling recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein affinity purification methods. Protein A (Cat. # 6500, Cat. # 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A than the other regular Protein-A Agarose on the market. The IgG binding capacity of Hi-BindTM Protein A-Agarose is ≥ 36 mg human or rabbit IgG per ml of wet beads. The Hi-BindTM Protein A-Agarose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.
  • Product Highlights
    • CONTENTS: Supplied as 50% slurry in 20% Ethanol/H2O. • FEATURES: - HIGH BINDING CAPACITY: Binding of IgG ≥ 36 mg human or rabbit IgG/ml Protein A-Agarose. - MINIMAL LEACHING OF THE LIGAND - FLOW RATE TESTED*: 2.89 ml/min *Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm). - MAXIMUM FLOW RATE* = 1800 cm/hr; minimum leaching of recombinant Protein A. NOTE * = the highest flow that beads withstand for 1 min, without collapsing and the pressure reaching 1 MPa. - USAGE: Reusable for up to 10 times without significant loss of binding capacity. • APPLICATIONS - Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. - Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
  • Storage Temp
    +4°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Notes
    For research use only. Not to be used for human or animal treatment or consumption.
  • Properties
    The purest agarose was used in the production of Hi-Bind™ Protein A-Agarose by Biovision.
  • Conjugation
    Agarose
  • Gene target
  • Short name
    Hi-Bind™ Protein A-Agarose
  • Technique
    Agarose, agaroses
  • Label
    Agarose
  • Alternative name
    Hi-Bind™ Protein A-molecular sieve
  • Alternative technique
    separation
MeSH Data
  • Name
  • Concept
    Scope note: Electrophoresis in which agar or agarose gel is used as the diffusion medium.
  • Tree numbers
    • E05.196.401.153
    • E05.301.300.100
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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