MHC Class 1, H2, KK (H-2 Class I Histocompatibility Antigen, K-K alpha Chain, H-2K(K), H2-K1, H2-K) (FITC) Antibody
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Catalog number
GEN603280
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Price
Please ask
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Size
500ug
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Also known as
MHC Class 1, H2, KK
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Other names
N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a,k
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Clone
36-7-5
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Host organism
Mouse (Mus musculus)
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Source organism
Mouse (Mus musculus)
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Reacts with the H-2Kk MHC class I alloantigen. Crossreactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis. Does not react with other (e.g., b, d, q) haplotypes.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Purified
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Form Appearance
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
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Description
The MHC Class 1, H2, KK (H-2 Class I Histocompatibility Antigen, K-K alpha Chain, H-2K(K), H2-K1, H2-K) (FITC) Antibody is a α- or alpha protein sometimes glycoprotein present in blood. Antigens are peptides or recombinant or native dependent on the production method.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
NCAPH2, ATP12A, TUBA3D, H4C13, NME2, CFB, TUBA1B, RAP2A
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Short name
MHC Class 1, H2, KK (H-2 Class I Histocompatibility Antigen, K-K alpha Chain, H-2K(K), H2-K1, H2-K) (FITC) Antibody
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Technique
Antibody, antigen, FITC, antibodies against human proteins, antibodies for, antigenes, Fluorescein
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Label
FITC
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Alternative name
Anti-MHC Class 1, H2, KK (H-2 Class I Histocompatibility Antigen, K-K alpha Chain, H-2K(K), H2-K1, H2-K) (FITC)
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Alternative technique
antibodies, antigenes, fluorescine
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Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
tubulin alpha 3d
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2007-02-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
H4 clustered histone 13
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Synonyms gene
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Synonyms gene name
- H4 histone family, member K
- histone 1, H4l
- histone cluster 1, H4l
- histone cluster 1 H4 family member l
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Synonyms
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GenBank acession
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Locus
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Discovery year
1998-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
complement factor B
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Synonyms gene
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- Complement system activation components
- Sushi domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene name
tubulin alpha 1b
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2007-02-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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