Phospho-p53 (Ser376) Antibody
-
Catalog number
A1158-100
-
Price
Please ask
-
Size
100 µl
-
-
Antibody Target
TP53
-
Target Alternative Name
P53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
-
Antibody Type
Polyclonal
-
Species Reactivities
Human, Mouse
-
Immunogen Sequence
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p53
-
Accession
P04637
-
Gene Id
7157
-
Appearance
Colorless liquid
-
Form
Liquid
-
Formulation
In 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol and 0.01% sodium azide
-
Purification
Affinity purified
-
Application
WB, IH
-
Positive Control
WB: NIH3T3, IHC: human brain tissue
-
Application And Usages
WB; 1:500 – 1:2000, IHC; 1:50 – 1:200
-
Description
p53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
-
Storage Condition
-20°C
-
Shipping Condition
gel pack
-
Shelf Life
12 months
-
Usage
For Research Use Only! Not to be used in humans
-
Handling
The antibody solution should be gently mixed before use
-
Western Blot Verified
Verified
-
Immunocytochemistry Verified
Verified
-
Immunofluorescence Verified
Not Verified
-
Immunoprecipitation Verified
Not Verified
-
FACS Verified
Not Verified
-
ELISA Verified
Not Verified
-
ChIP Verified
Not Verified
-
Dot Blot Verified
Not Verified
-
Flow Cytometry Verified
Not Verified
-
-
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
-
Gene target
-
Short name
Phospho-p53 (Ser376) Antibody
-
Technique
Antibody, antibodies against human proteins, antibodies for
-
Isotype
Rabbit IgG
-
Alternative name
phosphorilated-p53 (Ser376) (antibody to-)
-
Alternative technique
antibodies
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products