HGF, human recombinant

• Catalog number
  4510-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF
• Alternative_names
  Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF
• Description
  A growth factor regulating cell growth, cell motility, and morphogenesis
• Recombinant
  Yes
• Source
  CHO cells
• Purity by SDS PAGE
  ≥97%
• Assay
  SDS-PAGE
• Purity
  ≥97%
• Biological activity
  The ED₅₀, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.
• Results
  20-40 ng/ml.
• Molecular Weight
  80.017 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  The protein was lyophilized from a concentrated (1mg/ml) solution containing PBS pH 7.4 containing 0.05% Tween 20 and 100 mM L-arginine.
• Reconstitution Instructions
  It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor (HGF) in sterile 18MΩ-cm⁻¹ H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Background Information
  Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. HGF Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an α-chain of 463 amino acids and β-chain of 234 having a total molecular mass of 80.017 kDa. The HGF is purified by proprietary chromatographic techniques.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  HGF  
• Gene symbol
  SOS1, HGF, IL6
• Short name
  HGF, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  hepatocyte growth factor (hepapoietin A; scatter factor), H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  hepatocyte growth factor (hepapoietin A; scatter factor), DFNB39 and F-TCF and HGFB and HPTA and SF, HGF and IDBG-24150 and ENSG00000019991 and 3082, protein heterodimerization activity, Extracellular, Hgf and IDBG-136449 and ENSMUSG00000028864 and 15234, HGF and IDBG-631194 and ENSBTAG00000017664 and 282879

Gene info

• Identity
  HGNC:11187
• Gene
  SOS1
• Long gene name
  SOS Ras/Rac guanine nucleotide exchange factor 1
• Synonyms gene
  • GINGF
• Synonyms gene name
  • gingival fibromatosis, hereditary, 1
  • son of sevenless homolog 1 (Drosophila)
• Synonyms
  • HGF
  • GF1
• GenBank acession
  • L13857
• Locus
  2p22.1
• Discovery year
  1993-10-27
• Entrez gene record
  6654
• Pubmed identfication
  • 8276400
  • 10995566
• RefSeq identity
  • NM_005633
• Classification
  • Dbl family Rho GEFs
  • Pleckstrin homology domain containing
• VEGA ID
  OTTHUMG00000102109
• Locus Specific Databases
  • LRG_754

Gene info

• Identity
  HGNC:4893
• Gene
  HGF
• Long gene name
  hepatocyte growth factor
• Synonyms gene
  • DFNB39
• Synonyms gene name
  • deafness, autosomal recessive 39
  • hepatocyte growth factor (hepapoietin A; scatter factor)
• Synonyms
  • SF
  • F-TCF
  • HGFB
  • HPTA
• Synonyms name
  • hepatopoietin A
  • fibroblast-derived tumor cytotoxic factor
  • scatter factor
  • lung fibroblast-derived mitogen
• Locus
  7q21.11
• Discovery year
  1991-06-07
• Entrez gene record
  3082
• Pubmed identfication
  • 1837206
  • 19576567
• RefSeq identity
  • NM_000601
• VEGA ID
  OTTHUMG00000023804

Gene info

• Identity
  HGNC:6018
• Gene
  IL6
• Long gene name
  interleukin 6
• Synonyms gene
  • IFNB2
• Synonyms gene name
  • interleukin 6 (interferon, beta 2)
• Synonyms
  • IL-6
  • BSF2
  • HGF
  • HSF
• Synonyms name
  • interferon, beta 2
• GenBank acession
  • M18403
• Locus
  7p15.3
• Discovery year
  1986-01-01
• Entrez gene record
  3569
• Pubmed identfication
  • 3294161
• RefSeq identity
  • NM_000600
• Classification
  • Interleukins
  • Interferons
  • Interleukin 6 type cytokine family
• VEGA ID
  OTTHUMG00000023178

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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